ACE UHPLC and HPLC Columns
Conditions Analytes
Conditions Analytes
114
Conditions Analytes
2
3
1
Response - MilliVolts
cps
1.6e6
1.6e6
1.4e6
1.4e6
1.2e6
1.2e6
1.0e6
8.0e5
8.0e5
6.0e5
6.0e5
4.0e5
4.0e5
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com
Organotin Compounds
Application #AN3650
Column: ACE 3 C18
Dimensions: 150 x 2.1 mm
Part Number: ACE-111-1502
Mobile Phase: H2O/MeCN/acetic acid/TEA
(23:65:12:0.05 v/v/v/v)
Flow Rate: 0.2 mL/min
Detection: ICP-MS
1. Dibutyltin
2. Tributyltin
1
2
2 4 6 8 10 12
Reproduced with permission of LGC Ltd, Middlesex, UK
Dibutyltin
Tributyltin
OTC Gastric Drugs
Application #AN3940
Column: ACE 5 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: MeCN/10 mM ammonium
bicarbonate pH 8.0
in H2O (18:82)
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 254 nm
1. Famotidine
2. Cimetidine
3. Ranitidine
1 2 3 4 5 6 7 8 9 10 11 12
0
Time - Minutes Time - Minutes
Famotidine
Cimetidine Ranitidine
OH - oxysterol
Time - Minutes
Oxysterols by LC-MS/MS
Application #AN2380
Column: ACE 3 C18-AR
Dimensions: 150 x 2.1 mm
Part Number: ACE-119-1502
Mobile Phase: A: 0.1% formic acid in H2O/
MeOH (70:30 v/v)
B: 0.1% formic acid in MeOH
Gradient: Time (mins) %B
0.0 20
1.0 20
3.5 60
8.5 60
11.5 80
16.5 80
20.0 90
22.5 90
25.0 20
Flow Rate: 0.3 mL/min
Temperature: 40 °C
Detection: AB SCIEX API 4000 MS
Turbo IonSpray, positive
mode MRM
Sample: Derivatised with Girard
P reagent
1. 7α-Hydroxycholesterol
(m/z 534.4 455.4)
2. 7β-Hydroxycholesterol
(m/z 534.4 455.4)
3. 22(S)-Hydroxycholesterol
(m/z 534.4 455.4)
4. 22(R)-Hydroxycholesterol
(m/z 534.4 455.3)
5. 24(S)-Hydroxycholesterol
(m/z 534.5 455.4)
6. 25-Hydroxycholesterol
(m/z 534.4 455.4)
7. 27-Hydroxycholesterol
(m/z 534.4 455.4)
8. 7α,25-Dihydroxycholesterol
(m/z 550.4 471.4)
9. 7β,25-Dihydroxycholesterol
(m/z 550.4 471.4)
10. 7α,27-Dihydroxycholesterol
(m/z 550.4 471.4)
11. 7β,27-Dihydroxycholesterol
(m/z 550.4 471.4)
12. 3β,25-Dihydroxy-5-cholesten-7-one
(m/z 550.4 471.4)
13. 3β,27-Dihydroxy-5-cholesten-7-one
(m/z 550.4 471.4)
14. 5α,6α-Epoxycholestanol
(m/z 534.4 455.4)
13
6.0 8.0 10.0 12.0 14.0 16.0 18.0 20.0 mins
1.0e6
2.0e5
diOH-oxysterol
OH-oxysterol
Cholesterol
9
11
12
8
10
4 5
6
7
3
2
1 14
Cholesterol
diOH - oxysterol
1
2
3
4 7
11
12
13
14
0
6.0 8.0 10.0 12.0 14.0 16.0 18.0 20.0
2.0e5
Reproduced from supplement (pnas.org/content/suppl/2014/08/01/1322807111) to ‘Oxysterols are agonist ligands of RORγt and drive Th17 cell differentiation’,
PNAS, 111 (33), 12163-12168 (2014)