ACE UHPLC and HPLC Columns
Opiates from Drugs of Abuse Screen (#AN2190)
Application #AN2340
Conditions Analytes
1. Oxymorphone
(m/z 302.2 198.1)
2. Morphine-d3
(m/z 289.2 201.0)
3. Morphine
(m/z 286.2 201.0)
4. Hydromorphone
(m/z 286.2 185.1)
7. Dihydrocodeine
(m/z 302.2 199.1)
11. Oxycodone
(m/z 316.2 241.2)
15. 6-MAM
(m/z 328.2 165.1)
16. Codeine
(m/z 300.3 215.1)
19. Hydrocodone
(m/z 300.2 199.1)
25. EDDP
(m/z 278.2 234.2)
41. Methadone
(m/z 310.2 265.2)
1 2,3 4 7 11 1516 19 25 41
100
100
%
%
0
1 2,3 4 7 11 15 16 19 25 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0
mins
0
mins
8
8
7
7
6
6
5
4
3
2
GCounts
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Column: ACE Excel 1.7 C18
Dimensions: 100 x 2.1 mm
Part Number: EXL -171-1002U
Mobile Phase: A: 5 mM ammonium acetate in H2O
B: 5 mM ammonium acetate in MeOH
Gradient: Time (mins) %B
0.0 10
10.0 90
11.9 90
13.4 10
15.5 10
Flow Rate: 0.3 mL/min
Injection: 10 μL
Temperature: 40 °C
Detection: MS Quattro Premier XE triple quad
MRM, positive and negative ESI mode
Desolvation temperature: 450 °C
IonSource temperature: 150 °C
Collision gas pressure: 3.5 x 10-3 mbar
Reproduced with permission of Biotage GB Ltd
Time - Minutes
Opiates in Urine by LC-MS/MS
Morphine 3-b-D-glucuronide
2
1
3
4
TIC
1 2 3 4 5
1
0
1
1
1
0
2
2
2
3
3
3
4
4
4
5
5
5
Column: ACE Excel 3 SuperC18
Dimensions: 75 x 2.1 mm
Part Number: EXL -1111-7502U
Mobile Phase: A: 5 mM ammonium hydroxide pH 10.8
in H2O
B: 5 mM ammonium hydroxide pH 10.8
in MeOH/H2O (90:10 v/v)
Gradient: Time (mins) %B
0 30
5 95
Flow Rate: 0.6 mL/min
Injection: 2 μL
Temperature: 60 °C
Detection: Varian 320 Triple Quadrupole MS
Electrospray voltage: +5 kV
Inlet capillary voltage: 30 V
CID with argon at 1.5 mTorr
Collision cell potential ranges from 5 to 17 V
Drying gas (nitrogen) temperature: 325 °C
Nebulizing gas (nitrogen) pressure: 35 psi
Extended Dynamic Range
Application #AN1230
Conditions Analytes
1. Morphine 3-b-D-glucuronide
LOD (est) 100 ppb
(m/z 462.0 285.9)
2. Normorphine
LOD (est) 100 ppb
(m/z 272.0 165.0)
3. Morphine 6-b-D-glucuronide
LOD (est) 100 ppb
(m/z 462.0 285.9)
4. Morphine
LOD (est) 20 ppb
(m/z 286.0 200.9)
5. 6-Acetylmorphine
LOD (est) 10 ppb
(m/z 328.0 164.9)
Time - Minutes
TIC
Normorphine Morphine 6-b-D-glucuronide Morphine 6-Acetylmorphine