ACE UHPLC and HPLC Columns
ACE Bioanalytical 300 Å Method Development Kit
• Contains ACE C18-300, ACE C4-300 and ACE Phenyl-300 phases
• Ideal starting point for protein and peptide method development
• Available from microbore (0.5 mm id) through to analytical (4.6 mm id) dimensions
• Ultra-inert 300 Å phases provide excellent peak shape and reproducibility
Phase Functional Group
Particle
Size (μm)
Pore Size
(Å)
Surface
Area (m2/g)
Carbon Load
(%)
Recommended
pH Range
Explore
Selectivity
2 and 3 column kits
available for the
same price as a
single column
USP
Listing
ACE C18-300 Octadecyl (C18) 3, 5, 10 300 100 9.0 2.0-8.0 L1
ACE C4-300 Butyl (C4) 3, 5, 10 300 100 2.6 2.0-8.0 L26
ACE Phenyl-300 Phenyl 3, 5, 10 300 100 5.3 2.0-8.0 L11
ACE Bioanalytical 300 Å Method Development HPLC Column Kits
(Contains 3 columns: ACE C18-300, ACE C4-300 and ACE Phenyl-300 of specified dimensions)
(HPLC hardware format with 275 bar/4000 psi pressure limit)
Column Dimensions 3 μm 5 μm
2.1 x 20 mm MDKB-3-0202 MDKB-5-0202
2.1 x 30 mm MDKB-3-0302 MDKB-5-0302
2.1 x 35 mm MDKB-3-3502 MDKB-5-3502
2.1 x 50 mm MDKB-3-0502 MDKB-5-0502
2.1 x 75 mm MDKB-3-7502 MDKB-5-7502
2.1 x 100 mm MDKB-3-1002 MDKB-5-1002
2.1 x 125 mm MDKB-3-1202 MDKB-5-1202
2.1 x 150 mm MDKB-3-1502 MDKB-5-1502
2.1 x 250 mm - MDKB-5-2502
3.0 x 20 mm MDKB-3-0203 MDKB-5-0203
3.0 x 30 mm MDKB-3-0303 MDKB-5-0303
3.0 x 35 mm MDKB-3-3503 MDKB-5-3503
3.0 x 50 mm MDKB-3-0503 MDKB-5-0503
3.0 x 75 mm MDKB-3-7503 MDKB-5-7503
(HPLC hardware format with 275 bar/4000 psi pressure limit)
Column Dimensions 3 μm 5 μm
3.0 x 100 mm MDKB-3-1003 MDKB-5-1003
3.0 x 125 mm MDKB-3-1203 MDKB-5-1203
3.0 x 150 mm MDKB-3-1503 MDKB-5-1503
3.0 x 250 mm - MDKB-5-2503
4.6 x 20 mm MDKB-3-0246 MDKB-5-0246
4.6 x 30 mm MDKB-3-0346 MDKB-5-0346
4.6 x 35 mm MDKB-3-3546 MDKB-5-3546
4.6 x 50 mm MDKB-3-0546 MDKB-5-0546
4.6 x 75 mm MDKB-3-7546 MDKB-5-7546
4.6 x 100 mm MDKB-3-1046 MDKB-5-1046
4.6 x 125 mm MDKB-3-1246 MDKB-5-1246
4.6 x 150 mm MDKB-3-1546 MDKB-5-1546
4.6 x 250 mm - MDKB-5-2546
Note: 4.0 mm id ACE Bioanalytical 300 Å Method Development Kits also available – please enquire
ACE Bioanalytical 300 Å Method Development Microbore HPLC Column Kits
(Contains 3 columns: ACE C18-300, ACE C4-300 and ACE Phenyl-300 of specified dimensions)
(HPLC hardware format with 275 bar/4000 psi recommended pressure limit)
3 μm 5 μm
Column Dimensions 1/16” port 1/32” port 1/16” port 1/32” port
0.5 x 30 mm MDKB-3-03005 MDKB-3-03005S MDKB-5-03005 MDKB-5-03005S
0.5 x 50 mm MDKB-3-05005 MDKB-3-05005S MDKB-5-05005 MDKB-5-05005S
0.5 x 75 mm MDKB-3-75005 MDKB-3-75005S MDKB-5-75005 MDKB-5-75005S
0.5 x 100 mm MDKB-3-10005 MDKB-3-10005S MDKB-5-10005 MDKB-5-10005S
0.5 x 125 mm MDKB-3-12005 MDKB-3-12005S MDKB-5-12005 MDKB-5-12005S
0.5 x 150 mm MDKB-3-15005 MDKB-3-15005S MDKB-5-15005 MDKB-5-15005S
0.5 x 250 mm - - MDKB-5-25005 MDKB-5-25005S
1.0 x 30 mm MDKB-3-0301 MDKB-3-0301S MDKB-5-0301 MDKB-5-0301S
1.0 x 50 mm MDKB-3-0501 MDKB-3-0501S MDKB-5-0501 MDKB-5-0501S
1.0 x 75 mm MDKB-3-7501 MDKB-3-7501S MDKB-5-7501 MDKB-5-7501S
1.0 x 100 mm MDKB-3-1001 MDKB-3-1001S MDKB-5-1001 MDKB-5-1001S
1.0 x 125 mm MDKB-3-1201 MDKB-3-1201S MDKB-5-1201 MDKB-5-1201S
1.0 x 150 mm MDKB-3-1501 MDKB-3-1501S MDKB-5-1501 MDKB-5-1501S
1.0 x 250 mm - - MDKB-5-2501 MDKB-5-2501S
IMPORTANT NOTE: ACE microbore columns (1.0 mm id and 0.5 mm id) are available with either standard 1/16” (10-32 thread) connections or 1/32”
(6-40 thread) connections. For use with Eksigent micro and nano LC systems, order columns with 1/32” connections and use either ACE 6-40 fittings
(part number ACE-MC3210, 10 pack) or Eksigent 6-40 fittings (part number 5019621).
For 1/16” HPLC column connections up to 6000 psi, PEEK™ 1/16” fingertight fittings (part number ACE-CC10, 10 pack) are recommended. For 1/32” microbore HPLC column
connections up to 6000 psi, PEEK™ 1/32” (6-40 thread) fingertight fittings (part number ACE-MC3210, 10 pack) are recommended. For 1/16” UHPLC column connections up to
25000psi, reuseable 1/16” fittings (part number EXL-CC10, 10 pack) are recommended. To further extend UHPLC and HPLC column lifetimes,
ACE pre-column filters are recommended. For further details please contact your distributor or visit www.ace-hplc.com
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