ACE UHPLC and HPLC Columns
Conditions Analytes
8
10
8
9
11
9
12
10
13
11
14
12
15
13
14
15
ACE C18
ACE C18-PFP
0 1 2 3 4 5 6 7 8 9 150 11 12 13 14 15
dG
2000
1500
1000
Nucleosides and Vitamins
Conditions Analytes
0 1 2 3 4 5 6 7 8 9
Conditions Analytes
2000
2000
1500
1500
1000
1000
500
500
0
2000
UV (mv) UV (mv)
2000
1500
1500
1000
1000
500
Deoxyribonucleosides
dG
5
0 1 2 3 4 5 6 7 8
Ribonucleosides
9
ACE C18
0 1 2 3 4 5 6 7 8
500
0
Time - Minutes
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com 109
2000
1500
1000
500
0
dA
dA
dT
dT
dI
dI
dG
dU
dU
dC
dC
ACE C18
ACE C18-PFP
Time (min)
UV 260nm (mV)
0 1 2 3 4 5 6 7 8
0
ACE C18
ACE C18-PFP
Ado
Ado
Guo
Guo
Urd
Urd
Cyd
Cyd
Time (min)
Time - Minutes
2 3 4 5
min
1
2
4
5
8
9
Time - Minutes
Application #AN1330
Column: ACE 3 C18-PFP
Dimensions: 150 x 4.6 mm
Part Number: ACE-1110-1546
Mobile Phase: 20 mM H3PO4 in H2O
Flow Rate: 1 mL/min
Temperature: 22 °C
Detection: UV, 254 nm
1. Pyridoxamine (Vitamin B6)
2. Cytosine
3. Thiamine (Vitamin B1)
4. Nicotinamide
5. L -Ascorbic acid (Vitamin C)
6. Uracil
7. Cytidine
8. Hypoxanthine
9. 2-Deoxycytidine
2
4
3
6
7 1
0
6,7
6 7
5
5
4
4
3
3
2
2
1
1
500
Nucleic Acids / Disease Biomarker Profiling (II)
Application #AN1090
Column: ACE 3 C18-PFP
ACE 3 C18
Dimensions: 100 x 4.6 mm
Part Number: ACE-1110-1046, ACE-111-1046
Mobile Phase: 33 mM potassium
phosphate pH 6.2 with
KOH/MeOH (88:12 v/v)
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 260 nm
1. Deoxycytidine
2. Deoxyuridine
3. Deoxyinosine
4. Deoxyguanosine
5. Thymidine
6. Deoxyadenosine
7. Cytidine
8. Uridine
9. Guanosine
10. Adenosine
Reproduced with permission of Department of Medical Biochemistry and
Biophysics, Umeå University, Sweden
Nucleic Acids / Disease Biomarker Profiling (I)
Application #AN1080
Column: ACE 3 C18-PFP
ACE 3 C18
Dimensions: 100 x 4.6 mm
Part Number: ACE-1110-1046, ACE-111-1046
Mobile Phase: 33 mM potassium phosphate
pH 6.2 with KOH/MeOH
(88:12 v/v)
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 260 nm
1. dATP
2. dADP
3. dAMP
4. 5-Fluorodeoxyuridine
5. Adenine
6. Thymine
7. 2-Fluorodeoxyuridine
8. Adenine arabinoside
9. 2’-C-methyladenosine
10. Adenosine
11. Deoxyadenosine
12. Cordycepin
13. 2-Fluoroadenine
arabinoside
14. 2-Fluorodeoxyadenosine
15. 2-Fluoroadenosine
Reproduced with permission of Department of Medical Biochemistry and
Biophysics, Umeå University, Sweden
0 1 2 3 4 5 6 7 8 9
0
dA
dA
dT
dT
dI
dI
dG
dU
dU
dC
dC
ACE C18
ACE C18-PFP
Time (min)
UV 260nm (mV)
2000
1500
1000
500
ACE C18
ACE C18-PFP
ACE C18-PFP
UV (mV)
1
1
7
7
8
8
9
9
10
10
2
2
3
3
4
4
5
6
6