ACE UHPLC and HPLC Columns
Nitroanilines (I)
Conditions Analytes
Column: ACE 5 C18
Dimensions: 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: 50 mM KH2PO4 pH 3.15/
MeCN (50:50 v/v)
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 254 nm
Application #AN3240
1. p-Nitroaniline
2. m-Nitroaniline
3. o-Nitroaniline
p-Nitroaniline m-Nitroaniline o-Nitroaniline
1 2
3
2
p-Nitroaniline
Time - Minutes Time - Minutes
400
350
300
250
200
150
100
50
Nitrofuran Metabolites by LC-MS/MS
106
Nitroanilines (II)
350
300
250
200
150
100
50
0
Response - MilliVolts
Analytes
1. 5-Methylmorpholino-3-amino-2-oxazolidinone derivative (NBAMOZ)
(metabolite of furaltadone)
(m/z 335 291)
2. 3-Amino-2-oxazolidinone derivative (NBAOZ)
(metabolite of furazolidone)
(m/z 236 134)
3. 1-Aminohydantoin derivative (NBAHD)
(metabolite of nitrofurazone)
(m/z 249 134)
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Application #AN3050
Conditions
Reproduced with permission of Agricultural Ministry, Brazil
Column: ACE 5 CN
Dimensions: 250 x 4.6 mm
Part Number: ACE-124-2546
Mobile Phase: Heptane/Ethyl acetate (90:10 v/v)
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 254 nm
Application #AN3250
Conditions Analytes
1. p-Nitroaniline
2. m-Nitroaniline
3. o-Nitroaniline
1
3 2
6 2 6
m-Nitroaniline o-Nitroaniline
4 0 4 8 10 12 14
Column: ACE 3 C18
Dimensions: 50 x 2.1 mm
Part Number: ACE-111-0502
Mobile Phase: MeOH/0.5 mM ammonium
acetate in H2O (50:50 v/v)
Flow Rate: 0.2 mL/min
Injection: 20 μL
Temperature: Ambient
Detection: ESI MS/MS (+ve mode)
Sample: Metabolites derivatised with
2-nitrobenzaldehyde to form
nitrophenyl derivatives, prior to
LC-MS analysis
1
2
3
Time - Minutes
0
Response - MilliVolts