ACE UHPLC and HPLC Columns
EV
4.5
4.0
3.5
3.0
2.5
2.0
1.0
0.5
cps
7.5e5
7.5e5
7.0e5
7.0e5
6.5e5
6.5e5
6.0e5
6.0e5
5.5e5
5.5e5
5.0e5
5.0e5
4.5e5
4.5e5
4.0e5
4.0e5
3.5e5
3.5e5
3.0e5
3.0e5
2.5e5
2.5e5
2.0e5
2.0e5
1.5e5
1.5e5
1.0e5
1.0e5
5.0e4
5.0e4
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Mycotoxins by LC-MS/MS
Column: ACE Excel 2 C18-AR
Dimensions: 50 x 2.1 mm
Part Number: EXL -109-0502U
Mobile Phase: A: 1 mM ammonium acetate,
0.5% acetic acid in H2O
B: 1 mM ammonium acetate,
0.5% acetic acid in 95% MeOH
Gradient: Time (mins) %B
0.0 40
1.0 40
2.4 60
6.8 87
Flow Rate: 0.6 mL/min
Injection: 2 μL
Temperature: 40 °C
Detection: AB SCIEX triple quad 5500
Positive ESI mode
Source temperature: 500 °C
IonSpray voltage: 5500 V
Mycotoxins/Aflatoxins from Peppers
Column: ACE 3 C18-PFP
Dimensions: 150 x 4.6 mm
Part Number: ACE-1110-1546
Mobile Phase: H2O/MeOH (60:40 v/v)
Flow Rate: 1 mL/min
Injection: 100 μL
Temperature: 45 °C
Detection: Fluorescence, λEx 362 nm,
λEm 425 nm
Application #AN1200
Conditions Analytes
1. Aflatoxin G2
2. Aflatoxin G1
3. Aflatoxin B2
4. Aflatoxin B1
0.0 5.0 10.0 15.0 20.0 25.0 30.0
0.0
1
2
3
4
1.5
Time - Minutes
Aflatoxin G2 Aflatoxin G1
Aflatoxin B2 Aflatoxin B1
Reproduced with permission of Public Health Laboratory of Soria, Spain
Application #AN2330
Conditions Analytes
1. Aflatoxin G2
(m/z 331.1 313.1)
2. Aflatoxin G1
(m/z 329.0 243.1)
3. Aflatoxin B2
(m/z 315.1 287.0)
4. Aflatoxin B1
(m/z 313.1 285.0)
5. HT -2-toxin
(m/z 442.2 263.1)
6. T -2-toxin
(m/z 484.2 305.1)
7. Ochratoxin A
(m/z 404.1 239.0)
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5
0
min
cps
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5
Time - Minutes
0
1
2
3
4
5
6
7
Reproduced with permission of Biotage GB Ltd, UK