ACE UHPLC and HPLC Columns
mGluR5 PET Tracer by Radio HPLC Analysis
Application #AN2700
Conditions Analyte
Column: ACE 3 C18 1. 11C-ABP688
Dimensions: 50 x 4.6 mm
Part Number: ACE-111-0546
Mobile Phase: 0.1% TFA in H2O/MeCN (55:45 v/v)
Flow Rate: 1 mL/min
Injection: 20 μL
Detection: UV, 254 nm
Radio detection
x105
5
4
3
2
Intens
x105
2
1
0
x105
4
2
0
x105
4
Macrotetralides
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com 101
1
UV Detection
1.00
Radio Detection
1.00
-12
-13
-14
-15
-16
-17
0.00
30.0
20.0
10.0
0.0
0.00
2.00
2.00
3.00
3.00
4.00
4.00
1
Reproduced with permission of Centre of Radiopharmaceutical Science,
University Hospital Zurich, Switzerland
Microbial Extract by LC-MS
Application #AN1180
Conditions Analytes
Macrotetralides
1. Dinactin R1 = R3 = CH2CH3, R2 = R4 = CH3
2. Trinactin R1 = R2 = R3 = CH2CH3, R4 = CH3
3. Tetranactin R1 = R2 = R3 = R4 = CH2CH3
19 20 21 22 23 24 25 26
Time - Minutes
Time - Minutes
m/z
27
1
2
3
1
0
2
0
782.6936
787.6484
796.7134
801.6668
810.7294
815.6833
785 790 795 800 805 810 815
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 150 x 2.1 mm
Part Number: CORE-25A-1502U
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient: Time (mins) %B
0.0 5
5.0 5
20.0 100
25.0 100
Flow Rate: 0.2 mL/min
Detection: Dionex 3000RS UHPLC system
coupled with Bruker MaXis Q-TOF MS
Electrospray MS positive mode
Source end plate offset -500 V
Nebuliser gas (N2) 1.6 bar
Drying gas (N2) temp 180 °C
Collision energy 5.0 eV
Collision RF 600 Vpp
Reproduced with permission of Department of Chemistry, University of Warwick, UK
Marine Streptomyces extract
Dinactin
Trinactin
Tetranactin
For additional
column dimensions
Please enquire
email: info@ace-hplc.com